32 research outputs found
The Ursinus Weekly, December 23, 1904
Schaff anniversary • Alumni notes • Good usage • Forced to be a soldier • Notice • Rainy day dreamshttps://digitalcommons.ursinus.edu/weekly/2997/thumbnail.jp
Panel Discussion On The Clinical Management Of Blood Dyscrasias In The Older Age Groupâ€
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/111245/1/jgs00338.pd
Single-molecule studies of repressor–DNA interactions show long-range interactions
We have performed single-molecule studies of GFP–LacI repressor proteins bound to bacteriophage λ DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to be 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths